Protocols

alphavirus.org

The McInerney Laboratory at Karolinska Institutet, Stockholm, Sweden

Protocols

 

On this page we are collecting protocols, which we use regularly and are often asked to provide to collaborators and other colleagues. Please contact us if anything is unclear. Click on the links below for detailed descriptions of the assays and further reading.

 

Flow cytometry based assay for autophagic flux

 

Immunoprecipitation

 

Ribopuromycinylation staining

 

In vivo labeling of nascent viral RNA with BrUTP

 

Analysis of SG components using biotin-isoxazole fractionation

 

 

 

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