Protocols

alphavirus.org

The McInerney Laboratory at Karolinska Institutet, Stockholm, Sweden

Protocols


On this page we are collecting protocols, which we use regularly and are often asked to provide to collaborators and other colleagues. Please contact us if anything is unclear. Click on the links below for detailed descriptions of the assays and further reading.


Flow cytometry based assay for autophagic flux


Immunoprecipitation


Ribopuromycinylation staining


In vivo labeling of nascent viral RNA with BrUTP


Analysis of SG components using biotin-isoxazole fractionation